Technical and interpretative issues of fosfomycin susceptibility testing

Susceptibility of multiresistant gram-negative bacteria to fosfomycin and performance of different susceptibility testing methods.

Fosfomycin may be a treatment option for multiresistant Gram-negative bacteria. This study compared susceptibility methods using 94 multiresistant clinical isolates. With agar dilution (AD), susceptibilities were 81%, 7%, 96%, and 100% (CLSI) and 0%, 0%, 96%, and 30% (EUCAST), respectively, for Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Enterobacter spp. Categor...

Issues in antifungal susceptibility testing.

In line with the availability of an increasing array of systemic antifungal agents, there is a need for accurate, reproducible and predictive susceptibility testing of fungal isolates in order to help inform clinical choice. Much early attention on antifungal susceptibility testing focused on defining test parameters that produced reproducible and reliable intra- and inter-laboratory results an...

Antimicrobial susceptibility testing issues relating to EARSS

Susceptibility of urinary tract bacteria to fosfomycin.

We evaluated the in vitro activity of fosfomycin against urinary isolates in a region in Greece that exhibits considerable antimicrobial resistance by evaluating retrospectively relevant susceptibility data retrieved from the microbiological library of the University Hospital of Heraklion, Crete, Greece. We examined 578 urinary isolates. In total, 516 (89.2%) were susceptible to fosfomycin; 415...

Laboratory evaluation of the Anderson Technical semiautomated susceptibility testing system.

Anderson Technical, Inc. has developed rapid, semiautomated equipment for antimicrobial susceptibility testing. A total of 310 fresh clinical bacterial isolates (226 gram-negative and 75 gram-positive) were tested with the Anderson Technical system and compared with those of Micro-Media Systems, Inc. For the gram-positive organisms, 74.3% of the test pairs had identical minimum inhibitory conce...